Microsatellite loci isolation from river buffalo using enriched partial genomic libraries
نویسندگان
چکیده
منابع مشابه
Characterization of new Schistosoma mansoni microsatellite loci in sequences obtained from public DNA databases and microsatellite enriched genomic libraries.
In the last decade microsatellites have become one of the most useful genetic markers used in a large number of organisms due to their abundance and high level of polymorphism. Microsatellites have been used for individual identification, paternity tests, forensic studies and population genetics. Data on microsatellite abundance comes preferentially from microsatellite enriched libraries and DN...
متن کاملOptimized construction of microsatellite-enriched libraries.
The construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. Numerous protocols are available in the literature for the construction of these libraries; however, sometimes their low efficiency or lack of optimization in the protocols can restrict their ...
متن کاملMicrosatellite libraries enriched for several microsatellite sequences in plants.
Simple sequence repeats (SSRs), or microsatellites, have been isolated and characterized from numerous animal and plant species (6,7). These sequences have been shown to be highly polymorphic between individuals within populations or closely related genotypes. This, together with the fact that they can be converted into a simple polymerase chain reaction (PCR)based assay, has led to their wides...
متن کاملPolymorphic Microsatellite Loci Isolated from the Squalidus argentatus Using PCR-Based Isolation of Microsatellite Arrays (PIMA)
Squalidus argentatus (Sauvage and Dabry de Thiersant 1874) is a small-sized freshwater fish which is distributed in Mainland China, Hainan Island and Taiwan. The populations of S. argentatus have dropped sharply probably due to overharvesting and water pollution recently. Eleven polymorphic microsatellite markers were developed for the cyprinid fish S. argentatus. These new markers were tested ...
متن کاملConstructing gene-enriched plant genomic libraries using methylation filtration technology.
Full genome sequencing in higher plants is a very difficult task, because their genomes are often very large and repetitive. For this reason, gene targeted partial genomic sequencing becomes a realistic option. The method reported here is a simple approach to generate gene-enriched plant genomic libraries called methylation filtration. This technique takes advantage of the fact that repetitive ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Italian Journal of Animal Science
سال: 2007
ISSN: 1828-051X
DOI: 10.4081/ijas.2007.s2.338